Christopher Daniel Barnes

Peter Parker / Spider-Man (voice)

Sara Ballantine

Mary Jane Watson (voice)

Jennifer Hale

Felicia Hardy (voice)

Linda Gary

Aunt May Parker (voice)

Gary Imhoff

Harry Osborn (voice)

Ed Asner

J. Jonah Jameson (voice)

Rodney Saulsberry

Joe "Robbie" Robertson (voice)

Roscoe Lee Browne

Kingpin (voice)

Joseph Campanella

Dr. Curt Connors / Lizard (voice)

Maxwell Caulfield

Alistair Smythe (voice)

Majel Barrett

Anna Watson (voice)

Nick Jameson

Michael Morbius (voice)

Patrick Labyorteaux

Flash Thompson (voice)

Liz Georges

Debra Whitman (voice)

Neil Ross

Norman Osborn (voice)

Dawnn Lewis

Detective Terri Lee (voice)

David Warner

Landon (voice)

Efrem Zimbalist Jr.

Otto Octavius / Doctor Octopus (voice)

Joan Lee

Madame Web (voice)

John Beard

Newscaster (voice)